HB Inogadzira Paradigm Itsva Yekuchengetera Sample dzeBiological kuICL

Imperial College London (ICL) iri pamberi mukuferefeta kwesainzi uye, kuburikidza neDhipatimendi reImmunology and Inflammation neDhipatimendi reBrain Sciences, kuferefeta kwayo kunosanganisira rheumatology nehematology kusvika ku dementia, chirwere cheParkinson negomarara reuropi. Kutarisira kuferefeta kwakasiyana-siyana kwakadaro kunoda nzvimbo dzepamusoro-soro, kunyanya kuchengetera masampuli ehupenyu akakosha. Neil Galloway Phillipps, Senior Lab Manager wemadhipatimendi ese ari maviri, akaziva kudiwa kwemhinduro yekuchengetedza cryogenic inoshanda uye inogara kwenguva refu.

Zvinodiwa zveICL

1.Sisitimu yekuchengetera nitrogen yemvura ine simba guru uye yakabatanidzwa

2.Kuderedzwa kwekushandiswa kwenitrogen uye mari yekushandisa

3.Kuchengetedzwa kwemuenzaniso kwakavandudzwa uye kutevedzera mitemo

4.Nzira yakachengeteka uye inoshanda zvakanaka kune vaongorori

5.Mhinduro inogara kwenguva refu yekutsigira zvirongwa zvekuchengetedza nharaunda

Matambudziko

Dhipatimendi reImmunology reICL raimboshandisa nitrogen yemvura isina kusimba (LN) gumi nenhatu dzakasiyana.₂) matangi ekuchengetera mienzaniso yekuyedzwa kwekiriniki, masero esatellite uye masero ekutanga. Sisitimu iyi yakakamurwa yaitora nguva kuti igadziriswe, ichida kugara ichitariswa uye ichizadzwazve.

"Kuzadza matangi gumi nematatu kwakatora nguva yakawanda, uye kuchengeta zvese kwaive kwava kuoma zvakanyanya," akatsanangura Neil. "Yaive dambudziko rezvekufambisa, uye taida nzira inoshanda zvakanyanya yekutarisira nzvimbo yedu yekuchengetera zvinhu."

Mari yekuchengetedza matangi akawanda yaive imwe yedambudziko.₂kushandiswa kwakawanda kwaive kwakawedzera, zvichikonzera kukwira kwemari yekushandisa. Panguva imwe chete, kukanganisa kwezvakatipoteredza kwekuendeswa kwenitrogen kakawanda kwaive kwakasiyana nekuzvipira kwerabhoritari pakuchengetedza nharaunda. "Tave tichishanda kuti tiwane mibairo yakasiyana-siyana yekuchengetedza nharaunda, uye taiziva kuti kuderedza kushandiswa kwedu kwenitrogen kwaizoita mutsauko mukuru," akadaro Neil.

Kuchengetedzeka nekutevedzera mitemo zvaive zvinhu zvakakoshawo. Nematangi akawanda akapararira munzvimbo dzakasiyana-siyana, kutevera nzira dzekuwana nekuchengetedza zvinyorwa zvemazuva ano kwaive kwakaoma. "Zvakakosha kuti tizive chaizvo kuti ndiani ari kuwana mienzaniso, uye kuti zvese zvakachengetedzwa nemazvo zvichienderana nemitemo yeHuman Tissue Authority (HTA)," Neil akawedzera. "Sisitimu yedu yekare haina kuita kuti izvozvo zvive nyore."

Mhinduro yacho

ICL yaitova nemidziyo yakasiyana-siyana kubva kuHaier Biomedical - kusanganisira nzvimbo yekuchengetera zvinhu zvinotonhora, makabati ekuchengetedza zvinhu zvipenyu, CO₂ma incubator nema centrifuges - kukudziridza kuvimba nemhinduro dzekambani.

Saka Neil nechikwata chake vakataurirana neHaier Biomedical kuti vabatsire kugadzirisa matambudziko aya matsva, vachiisa CryoBio 43 LN ine simba guru.₂biobank yekubatanidza matangi ese gumi nematatu asingachinji kuita sisitimu imwe chete inoshanda zvakanyanya. Kuchinja kwacho kwaive nyore, nechikwata chaHaier chichitarisira kuiswa nekudzidzisa vashandi verabhoritari. Sisitimu itsva yakapinda muLN iripo.₂nzvimbo yacho ine zvigadziriso zvidiki chete. Nehurongwa hutsva huripo, kuchengetwa kwesampuro nekutarisira kwave kushanda zvakanaka zvikuru. "Chimwe chezvakanakira zvisingatarisirwi chaive chekuti takawana nzvimbo yakawanda sei," akadaro Neil. "Nekubviswa kwematangi ese ekare, tava nenzvimbo yakawanda murabhoritari yekuisa mimwe michina."

Kuchinja kuenda kunzvimbo yekuchengetera zvinhu zvine utsi kwakavandudza kuchengetedzeka uye nyore kushandisa. "Kare, pese pataiburitsa rack mutangi rine utsi, yaidonha nitrogen, iyo yaigara ichinetsa kuchengetedza. Iye zvino, nekuchengetera zvinhu zvine utsi, kwakachena uye kwakachengeteka kubata masampuli. Sisitimu ye biometric access yakasimbisawo kuchengetedzeka nekutevedzera mitemo nekuti tinogona kunyatsotevera kuti ndiani anopinda musystem uye riini."

Neil nechikwata chake vakaona kuti sisitimu iyi iri nyore kushandisa, nechirongwa chekudzidzisa chaHaier chaiita kuti vakwanise kushandisa vashandisi nekukurumidza.

Chinhu chisingatarisirwi asi chaigamuchirwa chaive matanho anodzoserwa otomatiki, izvo zvinoita kuti kupinda mutangi kuve nyore. "Nematangi ekare, vaongorori vaiwanzofanira kusimudza zvinhu zvakazara. Kunyangwe tangi idzva rakareba, matanho acho anobuda nekudzvanya bhatani, zvichiita kuti kuwedzera kana kubvisa masampuli kuve nyore kubata," akadaro Neil.

Kuchengetedza mienzaniso inokosha

Masampuleti akachengetwa muICL's cryogenic facility akakosha pakutsvaga kuri kuitwa. "Mamwe emasampuleti atinochengeta haatsiviwi zvachose," akadaro Neil.

"Tiri kutaura nezvekugadzirwa kwemasero machena eropa kubva kuzvirwere zvisingawanzoitiki, sampuro dzekuyedzwa kwekiriniki, nezvimwe zvinhu zvakakosha pakutsvagisa. Sampuro idzi hadzingoshandiswi murabhoritari chete; dzinogovaniswa nevanoshanda navo pasi rese, zvichiita kuti kuvimbika kwadzo kuve kwakakosha zvikuru. Kushanda kwemasero aya ndicho chinhu chikuru. Kana asina kuchengetwa zvakanaka, tsvakiridzo yaanotsigira inogona kukanganiswa. Ndosaka tichida nzvimbo inotonhorera yakavimbika zvikuru yatinogona kuvimba nayo. Nehurongwa hweHaier, tine rugare rwepfungwa rwakakwana. Tinogona kutarisa tembiricha chero nguva, uye kana tikaongororwa, tinogona kuratidza nechivimbo kuti zvese zvakachengetwa nemazvo."

 Kuvandudza kuchengetedzeka kwenzvimbo uye kushanda zvakanaka kwemari

Kuunzwa kwebhangi idzva rebiobank kwaderedza zvakanyanya kushandiswa kwenitrogen yemvura murabhoritari, kuchideredza kagumi. "Rimwe nerimwe rematangi ekare aya raitakura marita angangoita 125, saka kuabatanidza kwakaita mutsauko mukuru," Neil akatsanangura. "Iye zvino tiri kushandisa chidimbu chenitrogen yataishandisa kare, uye izvozvo zvinotipa kukunda kukuru mune zvemari uye zvakatipoteredza."

Nekuda kwekushaikwa kwenitrogen shoma, kuburitswa kwecarbon kwadzikira, zvichitsigira zvinangwa zverabhoritari zvekuchengetedza nharaunda. "Hazvisi zvenitrogen pachayo chete," Neil akawedzera. "Kushaikwa kwenitrogen shoma zvinoreva kuti marori mashoma ari mumugwagwa, uye simba shoma rinoshandiswa kugadzira nitrogen pakutanga." Kuvandudzwa uku kwakakosha zvekuti Imperial yakagamuchira mibairo yekuchengetedza nharaunda kubva kuLEAF neMy Green Lab vachitenda zvakaitwa.

Mhedziso

Bhangi reHaier Biomedical rekuchengetedza zvinhu rakachinja kugona kweICL kuchengetedza zvinhu, richivandudza kushanda zvakanaka, kuchengetedzeka uye kugara kwenguva refu ukuwo richideredza mitengo zvakanyanya. Nekutevedzera mitemo zviri nani, kuchengetedzeka kwemuenzaniso kwakawedzerwa uye kuderedzwa kwekukanganiswa kwezvakatipoteredza, kuvandudzwa uku kwave kubudirira zvikuru.

Mhedzisiro yeProjekiti

1.LN₂kushandiswa kwemafuta kwakadzikiswa ne90%, zvichideredza mitengo uye utsi hunobuda

2.Kutevera sampuro kunoshanda zvakanyanya uye kutevedzera HTA

3.Kuchengetedzwa kwakachengeteka kwechikamu chemhepo kune vaongorori

4.Kuwedzera kugona kwekuchengetera zvinhu musystem imwe chete

5.Kurumbidzwa kuburikidza nemibairo yekuchengetedza nharaunda