HB Inogadzira Paradigm Itsva yeBiological Sample Storage paICL

Imperial College London (ICL) iri pamberi pekuferefeta kwesainzi uye, kuburikidza neDhipatimendi reImmunology uye Kuzvimba uye neDhipatimendi reBrain Sayenzi, tsvakiridzo yaro inotangira kubva kurheumatology uye hematology kusvika kudementia, chirwere cheParkinson negomarara reuropi. Kutarisira kutsvagisa kwakasiyana kwakadaro kunoda zvivakwa zvechizvino-zvino, kunyanya zvekuchengetedza akakosha masampuli ebhayoloji. Neil Galloway Phillipps, Senior Lab Maneja wemadhipatimendi ese ari maviri, akaziva kukosha kweimwe inoshanda uye yakagadzikana cryogenic yekuchengetedza mhinduro.

ICL Inoda

1.A high-capacity, consolidated liquid nitrogen storage system

2.Yakaderedzwa nitrogen yekushandisa uye mari yekushandisa

3.Kuvandudzwa kwekuchengetedza sampuli uye kutevedzera mutemo

4.Kuwanikwa kwakachengeteka uye kwakanyanya kune vaongorori

5.Mhinduro yakagadzikana yekutsigira zvirongwa zvegirinhi

Matambudziko

ICL's Dhipatimendi reImmunology raimbovimba negumi netatu rakasiyana static liquid nitrogen (LN₂) matangi ekuchengetedza makiriniki ekuedza samples, satellite maseru uye maprimary cell tsika. Iyi yakakamurwa sisitimu yaitora nguva kuchengetedza, ichida kugara ichitariswa uye kuzadzazve.

“Kuzadza matangi 13 kwakatora nguva yakawanda, uye kuchengeta chinyorwa chezvose kwakanga kwava kuoma zvikuru,” akatsanangura kudaro Neil. "Rakanga riri dambudziko rekugadzirisa zvinhu, uye taida imwe nzira inoshanda yekuchengetedza chengetedzo yedu."

Mutengo wekuchengetedza matangi akawanda yaive imwe nyaya yainetsa. LN₂kunwa kwakawanda, zvichikonzera kukwira kwemari yekushandisa. Panguva imwe cheteyo, kukanganisa kwezvakatipoteredza kwekugaro unza nitrogen kwaipesana nekuzvipira kwerabhoritari mukuenderera mberi. "Tanga tichishanda kuti tiwane mibairo yakasiyana-siyana, uye taiziva kuti kuderedza kushandiswa kwedu kwenitrogen kwaizoita mutsauko mukuru," Neil akadaro.

Chengetedzo uye kutevedza zvainyanya kukosha. Nematangi akawanda akapararira munzvimbo dzakasiyana-siyana, kutsvaga kuwana uye kuchengetedza zvinyorwa zvemazuva ano zvaive zvakaoma. “Zvakakosha kuti tinyatsoziva kuti ndiani ari kuwana masamples aya, uye kuti zvinhu zvese zvichengetwe nemazvo zvichifambirana nemitemo yeHuman Tissue Authority (HTA),” akawedzera Neil. "Maitiro edu ekare haana kuita kuti zvive nyore."

Mhinduro

ICL yanga yatove nemhando yemidziyo kubva kuHaier Biomedical - inotenderera inotonhora yekuchengetedza, biological kuchengetedza makabati, CO₂incubators uye centrifuges - kukudziridza kuvimba mumhinduro dzekambani.

Neil nechikwata chake saka vakaenda kuHaier Biomedical kuti vabatsire kugadzirisa matambudziko matsva aya, nekuisa yakakura-inokwana CryoBio 43 LN.₂biobank yekubatanidza ese gumi nematatu static matangi kuita imwechete yakakwirira - inoshanda sisitimu. Shanduko iyi yaive isina musono, nechikwata chaHaier chinodzora kuisirwa nekudzidzisa vashandi vemurabhoritari. Iyo itsva sisitimu yakapinda muLN iripo₂nzvimbo ine zvigadziriso zvidiki chete. Nehurongwa hutsva huripo, sampuli yekuchengetedza uye manejimendi zvave zvakanyanya kushanda zvakanaka. “Chimwe chezvakanakira zvatakanga tisingatarisiri ndechekuti takawana nzvimbo yakawanda sei,” akadaro Neil. "Nematangi ese ekare akabviswa, tave nenzvimbo yakawanda murabhoritari yemimwe michina."

Kuchinja kuenda kumhute-chikamu chekuchengetedza kwawedzera kuchengetedzeka uye nyore kushandisa. "Kare, pese patakabudisa raki kubva mutangi remvura-chikamu, inenge ichidonha nenitrogen, iyo yaigara ichinetsa kuchengetedza." Ikozvino, nekuchengetedza-chikamu cheupfu, kwakachena uye kwakachengeteka kubata masample.

Neil nechikwata chake vakawana iyo system intuitive kushandisa, neHaier's dzidziso chirongwa ichivagonesa kukurumidza kukwira vashandisi vekupedzisira.

Chinhu chisingatarisirwi asi chinogamuchirwa chaive matanho ekudzoreredza otomatiki, anoita kuti kuwana tangi kuve nyore. "Nematangi ekare, vatsvakurudzi vaiwanzosimudza zvinhu kunze zvakakwana. Kunyange zvazvo tangi itsva iri kureba, matanho anoiswa pakubaya bhatani, zvichiita kuti kuwedzera kana kubvisa masampuli kuve nyore kubata," Neil akadaro.

Kuchengetedza sampuli dzakakosha

Iwo masampula akachengetwa muICL's cryogenic nzvimbo akakosha pakuenderera mberi nekutsvaga. "Mamwe emasampuli atinochengeta haagadziriswe zvachose," akadaro Neil.

"Tiri kutaura nezvekugadzirira kwemasero machena eropa kubva kuzvirwere zvisingawanzoitiki, sampuli dzekiriniki, uye zvimwe zvinhu zvakakosha pakutsvaga. Masampuli aya haangoshandiswi murabhoritari chete; anogovaniswa nevanoshanda navo pasi rese, izvo zvinoita kuti kuvimbika kwavo kuve kwakakosha. Kushanda kwemaseru aya chinhu chose. Kana akasachengetwa nemazvo, tsvakiridzo yaanotsigira inogona kukanganisa. Tinokwanisa kutarisa mamiriro ekushisa chero nguva, uye kana tikamboongororwa, tinogona kuratidza nechivimbo kuti zvese zvakachengetwa nemazvo.

 Kuvandudza kusimba uye kushandiswa kwemari

Kuunzwa kweiyo biobank nyowani kwakaderedza zvakanyanya kushandiswa kwemvura yenitrogen, kuchicheka kagumi. “Tangi rimwe nerimwe rekare raitakura marita angangoita 125, saka kuabatanidza kwaunza mutsauko mukuru,” Neil akatsanangura. "Isu tave kushandisa chidimbu chenitrogen yatakamboita, uye iko kuhwina kukuru mune zvemari uye mune zvakatipoteredza."

Nemashoma ekuunza nitrogen anodiwa, kabhoni emissions yakaderedzwa, zvichitsigira zvibodzwa zverabhoritari. "Haisi chete nezve nitrogen pachayo," Neil akawedzera. "Kuva nekutakura zvishoma zvinoreva marori mashoma mumugwagwa, uye simba shoma riri kushandiswa kugadzira nitrogen pakutanga." Kuvandudzwa uku kwaive kwakakosha zvekuti Imperial yakagamuchira mibairo yekusimudzira kubva kune ese LEAF uye My Green Lab mukucherechedza kuedza kwayo.

Mhedziso

Haier Biomedical's cryogenic biobank yakashandura hunyanzvi hwekuchengetedza ICL, kuvandudza kugona, kuchengetedzeka uye kusimba uku ichidzikisa zvakanyanya mutengo. Nekuteedzera kurinani, kukwidziridzwa kwekuchengetedza sampuli uye kuderedzwa kwemamiriro ekunze, kukwidziridzwa kwave kuri budiriro inokatyamadza.

Project Outcomes

1.LN₂kushandiswa kwakaderedzwa ne90%, kuderedza mari uye kubuda

2.Yakanyanya kushanda sampuli yekutevera uye kutevedzera HTA

3.Safer vapour-phase kuchengetedza kune vanoongorora

4.Kuwedzera kuchengetedza kugona mune imwechete system

5.Kuzivikanwa kuburikidza nekusimudzira mibairo